Luteinizing Hormone Surge-Induced Kruppel-like Factor 4 Inhibits Cyp17A1 Expression in Preovulatory Granulosa Cells.

ABSTRACT: Previous in vivo and in vitro studies have demonstrated a dramatic up-regulation of Krüppel-like factor 4 (Klf4) in rat preovulatory granulosa cells (GCs) after LH/hCG treatment and its role in regulating Cyp19A1 expression during the luteal shift in steroidogenesis. In this study, we examined whether Klf4 also mediates the LH-induced repression of Cyp17A1 expression in primary rat preovulatory GCs. In response to LH treatment of GCs in vitro, Cyp17A1 expression declined to less than half of its initial value by 1 h, remaining low for 24 h of culture. Overexpression of Klf4 decreased basal and Sf1-induced Cyp17A1 expressions and increased progesterone secretion. Reduction of endogenous Klf4 by siRNA elevated basal Cyp

SUBMITTER: Choi Y 

PROVIDER: S-EPMC10813437 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature