The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening.

ABSTRACT: Problems with the male reproductive system are of both medical and social significance. As a rule, spermatozoa and seminal plasma proteomes are investigated separately to assess sperm quality. The current study aimed to compare ejaculate proteomes with spermatozoa and seminal plasma protein profiles regarding the identification of proteins related to fertility scores. A total of 1779, 715, and 2163 proteins were identified in the ejaculate, seminal plasma, and spermatozoa, respectively. Among these datasets, 472 proteins were shared. GO enrichment analysis of the common proteins enabled us to distinguish biological processes such as single fertilization (GO:0007338), spermatid development (GO:0007286), and cell motility (GO:0048870). Among the abundant terms for GO cellular components, zon

SUBMITTER: Shkrigunov T 

PROVIDER: S-EPMC10813512 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature