Inflammation and Starvation Affect Housekeeping Gene Stability in Adipose Mesenchymal Stromal Cells.

ABSTRACT: Due to the scientific success of in vitro and in vivo model studies, the interest in using mesenchymal stromal cells (MSCs) for the treatment of orthopaedic conditions is growing. In the context of osteoarthritis (OA), MSCs, and, in particular, those derived from adipose tissues (ASCs), have found broader access to clinical use as active components of minimally manipulated orthobiologics, as well as clinically expanded cell preparations, or to collect their released factors (secretome) for cell-free approaches. In this regard, while both inflammatory priming and starvation are common strategies used to empower cell potency or collect the secretome, respectively, little is known about the possible influence of these approaches on the stability of housekeeping genes (HKGs) for molecular stud

SUBMITTER: Ragni E 

PROVIDER: S-EPMC10814131 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature