The Proteomic Composition and Organization of Constitutive Heterochromatin in Mouse Tissues.

ABSTRACT: Pericentric heterochromatin (PCH) forms spatio-temporarily distinct compartments and affects chromosome organization and stability. Albeit some of its components are known, an elucidation of its proteome and how it differs between tissues in vivo is lacking. Here, we find that PCH compartments are dynamically organized in a tissue-specific manner, possibly reflecting compositional differences. As the mouse brain and liver exhibit very different PCH architecture, we isolated native PCH fractions from these tissues, analyzed their protein compositions using quantitative mass spectrometry, and compared them to identify common and tissue-specific PCH proteins. In addition to heterochromatin-enriched proteins, the PCH proteome includes RNA/transcription and membrane-related proteins, which show

SUBMITTER: Schmidt A 

PROVIDER: S-EPMC10814525 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature