Functional Classification and Interaction Selectivity Landscape of the Human SH3 Domain Superfamily.

ABSTRACT: SRC homology 3 (SH3) domains are critical interaction modules that orchestrate the assembly of protein complexes involved in diverse biological processes. They facilitate transient protein-protein interactions by selectively interacting with proline-rich motifs (PRMs). A database search revealed 298 SH3 domains in 221 human proteins. Multiple sequence alignment of human SH3 domains is useful for phylogenetic analysis and determination of their selectivity towards PRM-containing peptides (PRPs). However, a more precise functional classification of SH3 domains is achieved by constructing a phylogenetic tree only from PRM-binding residues and using existing SH3 domain-PRP structures and biochemical data to determine the specificity within each of the 10 families for particular PRPs. In additi

SUBMITTER: Kazemein Jasemi NS 

PROVIDER: S-EPMC10814857 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature