Quantitative Determination of Aflatoxin B₁ in Maize and Feed by ELISA and Time-Resolved Fluorescent Immunoassay Based on Monoclonal Antibodies.

ABSTRACT: In this study, a highly sensitive monoclonal antibody (mAb) was developed for the detection of aflatoxin B₁ (AFB₁) in maize and feed. Additionally, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and time-resolved fluorescence immunoassay assay (TRFICA) were established. Firstly, the hapten AFB₁-CMO was synthesized and conjugated with carrier proteins to prepare the immunogen for mouse immunization. Subsequently, mAb was generated using the classical hybridoma technique. The lowest half-maximal inhibitory concentration (IC50) of ic-ELISA was 38.6 ng/kg with a linear range of 6.25-100 ng/kg. The limits of detections (LODs) were 6.58 ng/kg and 5.54 ng/kg in maize and feed, respectively, with the recoveries ranging from 72% to 94%. The TRFICA

SUBMITTER: Han S 

PROVIDER: S-EPMC10815167 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature