Bacteriophage T5 dUTPase: Combination of Common Enzymatic and Novel Functions.

ABSTRACT: The main function of dUTPases is to regulate the cellular levels of dUTP and dTTP, thereby playing a crucial role in DNA repair mechanisms. Despite the fact that mutant organisms with obliterated dUTPase enzymatic activity remain viable, it is not possible to completely knock out the dut gene due to the lethal consequences of such a mutation for the organism. As a result, it is considered that this class of enzymes performs an additional function that is essential for the organism's survival. In this study, we provide evidence that the dUTPase of bacteriophage T5 fulfills a supplemental function, in addition to its canonical role. We determined the crystal structure of bacteriophage T5 dUTPase with a resolution of 2.0 Å, and we discovered a distinct short loop consisting of six amin

SUBMITTER: Glukhov A 

PROVIDER: S-EPMC10815766 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature