CRISPR/Cas9-Mediated Mutagenesis of Antennapedia in Spodoptera frugiperda.

ABSTRACT: The homeotic gene Antennapedia (Antp) has been identified as playing a pivotal role in the morphogenesis of the thorax and wings across various insect species. Leveraging insights from previous studies, the functional characterization of Antp in S. frugiperda was undertaken using RT-qPCR and the CRISPR/Cas9 genome-editing system. Phylogenetic analyses indicate that Antp shares a high degree of sequence homology among Lepidoptera species. The expression profile of SfAntp was detected by RT-qPCR. The results showed that SfAntp was expressed in the whole growth cycle of S. frugiperda, the expression level was the highest in the egg stage, and the expression level was higher from 12 h to 48 h. Tissue-specific expression profiling demonstr

SUBMITTER: Wang C 

PROVIDER: S-EPMC10816051 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature