Calciprotein Particles Induce Cellular Compartment-Specific Proteome Alterations in Human Arterial Endothelial Cells.

ABSTRACT: Calciprotein particles (CPPs) are indispensable scavengers of excessive Ca²⁺ and PO₄^3- ions in blood, being internalised and recycled by liver and spleen macrophages, monocytes, and endothelial cells (ECs). Here, we performed a pathway enrichment analysis of cellular compartment-specific proteomes in primary human coronary artery ECs (HCAEC) and human internal thoracic artery ECs (HITAEC) treated with primary (amorphous) or secondary (crystalline) CPPs (CPP-P and CPPs, respectively). Exposure to CPP-P and CPP-S induced notable upregulation of: (1) cytokine- and chemokine-mediated signaling, Ca²⁺-dependent events, and apoptosis in cytosolic and nuclear proteomes; (2) H⁺ and Ca²⁺ transmembrane transport, generation of reactive

SUBMITTER: Shishkova D 

PROVIDER: S-EPMC10816121 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature