Proteome Analysis of Serum Purified Using Solanum tuberosum and Lycopersicon esculentum Lectins.

ABSTRACT: Serum and plasma exhibit a broad dynamic range of protein concentrations, posing challenges for proteome analysis. Various technologies have been developed to reduce this complexity, including high-abundance depletion methods utilizing antibody columns, extracellular vesicle enrichment techniques, and trace protein enrichment using nanobead cocktails. Here, we employed lectins to address this, thereby extending the scope of biomarker discovery in serum or plasma using a novel approach. We enriched serum proteins using 37 different lectins and subjected them to LC-MS/MS analysis with data-independent acquisition. Solanum tuberosum lectin (STL) and Lycopersicon esculentum lectin (LEL) enabled the detection of more serum proteins than the other lectins. STL and LEL bind to N-ace

SUBMITTER: Nakajima D 

PROVIDER: S-EPMC10816257 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature