Characterization, Bioactivity, and Biodistribution of 35 kDa Hyaluronan Fragment.

ABSTRACT: It has been reported that hyaluronic acid (HA) with a 35 kDa molecular weight (HA35) acts biologically to protect tissue from injury, but its biological properties are not yet fully characterized. This study aimed to evaluate the cellular effects and biodistribution of HA35 compared to HA with a 1600 kDa molecular weight (HA1600). We assessed the effects of HA35 and HA1600 on cell migration, NO and ROS generation, and gene expression in cultured macrophages, microglia, and lymphocytes. HA35 was separately radiolabeled with ^99mTc and ¹²⁵I and administered to C57BL/6J mice for in vivo biodistribution imaging. In vitro studies indicated that HA35 and HA1600 similarly enhanced cell migration through HA receptor binding mechanisms, reduced the generation of NO and ROS, and

SUBMITTER: Gantumur MA 

PROVIDER: S-EPMC10817694 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature