A renal clearable fluorogenic probe for in vivo β-galactosidase activity detection during aging and senolysis.

ABSTRACT: Accumulation of senescent cells with age leads to tissue dysfunction and related diseases. Their detection in vivo still constitutes a challenge in aging research. We describe the generation of a fluorogenic probe (sulfonic-Cy7Gal) based on a galactose derivative, to serve as substrate for β-galactosidase, conjugated to a Cy7 fluorophore modified with sulfonic groups to enhance its ability to diffuse. When administered to male or female mice, β-galactosidase cleaves the O-glycosidic bond, releasing the fluorophore that is ultimately excreted by the kidneys and can be measured in urine. The intensity of the recovered fluorophore reliably reflects an experimentally controlled load of cellular senescence and correlates with age-associated anxiety during aging and senolytic treatment. Interest

SUBMITTER: Rojas-Vazquez S 

PROVIDER: S-EPMC10817927 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature