Patient-Derived Lung Cancer "Sandwich Cultures" with a Preserved Tumor Microenvironment.

ABSTRACT: In the past, different spheroid-, organotypic-, and three-dimensional (3D) bioprinting lung cancer models were established for in vitro drug testing and personalized medicine. These tissue models cannot depict the tumor microenvironment (TME) and, therefore, research addressing tumor cell-TME interactions is limited. To overcome this hurdle, we applied patient-derived lung tumor samples to establish new in vitro models. To analyze the tissue model properties, we established two-dimensional (2D) and 3D coculture tissue models exposed to static and dynamic culture conditions that afforded tissue culture for up to 28 days. Our tissue models were characterized by hematoxylin eosin staining, M30 enzyme-linked immunosorbent assay, and immunofluorescence staining against specific lu

SUBMITTER: Wang H 

PROVIDER: S-EPMC10818046 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature