Exploring Genomics and Microbial Ecology: Analysis of Bidens pilosa L. Genetic Structure and Soil Microbiome Diversity by RAD-Seq and Metabarcoding.

ABSTRACT: Bidens pilosa L., native to South America and commonly used for medicinal purposes, has been understudied at molecular and genomic levels and in its relationship with soil microorganisms. In this study, restriction site-associated DNA markers (RADseq) techniques were implemented to analyze genetic diversity and population structure, and metabarcoding to examine microbial composition in soils from Palmira, Sibundoy, and Bogotá, Colombia. A total of 2,984,123 loci and 3485 single nucleotide polymorphisms (SNPs) were identified, revealing a genetic variation of 12% between populations and 88% within individuals, and distributing the population into three main genetic groups, F_ST = 0.115 (p < 0.001) and F_IT = 0.013 (p > 0.05). In the soil analysis, si

SUBMITTER: Reyes-Ardila WL 

PROVIDER: S-EPMC10818919 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature