RNA-Seq of Phenotypically Distinct <i>Eimeria maxima</i> Strains Reveals Coordinated and Contrasting Maturation and Shared Sporogonic Biomarkers with <i>Eimeria acervulina</i>.

ABSTRACT: Strains of Eimeria maxima, an enteric parasite of poultry, vary in virulence. Here, we performed microscopy and RNA sequencing on oocysts of strains APU-1 (which exhibits more virulence) and APU-2. Although each underwent parallel development, APU-1 initially approached maturation more slowly. Each strain sporulated by hour 36; their gene expression diverged somewhat thereafter. Candidate biomarkers of viability included 58 genes contributing at least 1000 Transcripts Per Million throughout sporulation, such as cation-transporting ATPases and zinc finger domain-containing proteins. Many genes resemble constitutively expressed genes also important to Eimeria acervulina. Throughout sporulation, the expression of only a few genes differed between strains; these included cyclophi

SUBMITTER: Tucker MS 

PROVIDER: S-EPMC10818985 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature