Lactoferrin and Lysozyme Inhibit the Proteolytic Activity and Cytopathic Effect of <i>Naegleria fowleri</i> Enzymes.

ABSTRACT: Naegleria fowleri is a ubiquitous free-living amoeba that causes primary amoebic meningoencephalitis. As a part of the innate immune response at the mucosal level, the proteins lactoferrin (Lf) and lysozyme (Lz) are secreted and eliminate various microorganisms. We demonstrate that N. fowleri survives the individual and combined effects of bovine milk Lf (bLf) and chicken egg Lz (cLz). Moreover, amoebic proliferation was not altered, even at 24 h of co-incubation with each protein. Trophozoites' ultrastructure was evaluated using transmission electron microscopy, and these proteins did not significantly alter their organelles and cytoplasmic membranes. Protease analysis using gelatin-zymograms showed that secreted proteases of N. fowleri were differentially modulated b

SUBMITTER: Martinez-Castillo M 

PROVIDER: S-EPMC10819050 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature