Using Electroporation to Improve and Accelerate Zebrafish Embryo Toxicity Testing.

ABSTRACT: Zebrafish have emerged as a useful model for biomedical research and have been used in environmental toxicology studies. However, the presence of the chorion during the embryo stage limits cellular exposure to toxic elements and creates the possibility of a false-negative or reduced sensitivity in fish embryo toxicity testing (FET). This paper presents the use of electroporation as a technique to improve the delivery of toxic elements inside the chorion, increasing the exposure level of the toxins at an early embryo stage (<3 h post-fertilization). A custom-made electroporation device with the required electrical circuitry has been developed to position embryos between electrodes that provide electrical pulses to expedite the entry of molecules inside the chorion. The optimized parameters

SUBMITTER: Tazin N 

PROVIDER: S-EPMC10819337 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature