Genomic Analysis of a Community-Acquired Methicillin-Resistant <i>Staphylococcus aureus</i> Sequence Type 1 Associated with Caprine Mastitis.

ABSTRACT: This study aimed to investigate the genomic and epidemiological features of a methicillin-resistant Staphylococcus aureus sequence type 1 (MRSA ST1) strain associated with caprine subclinical mastitis. An S. aureus strain was isolated from goat's milk with subclinical mastitis in Paraiba, Northeastern Brazil, by means of aseptic procedures and tested for antimicrobial susceptibility using the disk-diffusion method. Whole genome sequencing was performed using the Illumina MiSeq platform. After genome assembly and annotation, in silico analyses, including multilocus sequence typing (MLST), antimicrobial resistance and stress-response genes, virulence factors, and plasmids detection were performed. A comparative SNP-based phylogenetic analysis was performed using publicly availa

SUBMITTER: Vasconcelos PC 

PROVIDER: S-EPMC10819347 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature