Simultaneous Two- and Three-Photon Deep Imaging of Autofluorescence in Bacterial Communities.

ABSTRACT: The intrinsic fluorescence of bacterial samples has a proven potential for label-free bacterial characterization, monitoring bacterial metabolic functions, and as a mechanism for tracking the transport of relevant components through vesicles. The reduced scattering and axial confinement of the excitation offered by multiphoton imaging can be used to overcome some of the limitations of single-photon excitation (e.g., scattering and out-of-plane photobleaching) to the imaging of bacterial communities. In this work, we demonstrate in vivo multi-photon microscopy imaging of Streptomyces bacterial communities, based on the excitation of blue endogenous fluorophores, using an ultrafast Yb-fiber laser amplifier. Its parameters, such as the pulse energy, duration, wavelength, and repetition rate,

SUBMITTER: Fernandez A 

PROVIDER: S-EPMC10819415 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature