Regulatory Role of GgaR (YegW) for Glycogen Accumulation in Escherichia coli K-12.

ABSTRACT: Glycogen, the stored form of glucose, accumulates upon growth arrest in the presence of an excess carbon source in Escherichia coli and other bacteria. Chromatin immunoprecipitation screening for the binding site of a functionally unknown GntR family transcription factor, YegW, revealed that the yegTUV operon was a single target of the E. coli genome. Although none of the genes in the yegTUV operon have a clear function, a previous study suggested their involvement in the production of ADP-glucose (ADPG), a glycogen precursor. Various validation through in vivo and in vitro experiments showed that YegW is a single-target transcription factor that acts as a repressor of yegTUV, with an intracellular concentration of consistently approximately 10 molecules,

SUBMITTER: Saito S 

PROVIDER: S-EPMC10819704 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature