Effect of photobiomodulation on inflammatory cytokines produced by HaCaT keratinocytes.

ABSTRACT:

Objective

to evaluate the effects of the red and near-infrared wavelength lasers in isolated and simultaneous way on the modulation of inflammatory cytokines produced by human keratinocytes (HaCaT) challenged by cytokines of human monocytes stimulated by lipopolysaccharide from Escherichia coli.

Design

HaCaT cells was previously exposed to the laser with wavelengths red (660 nm), near-infrared (808 nm). Then, HaCat cells were stimulated with the supernatant of lipopolysaccharide-challenged peripheral blood cells. The cytokines expressed by HaCat cells were measured using multiplex CBA assay.

Results

HaCaT cells increased the production of inflammatory cytokines when stimulated with infrared laser compared to the control group (IFN-α2, IFN-γ, TNF-α, MCP-1, IL-6,

SUBMITTER: Sa MGRS 

PROVIDER: S-EPMC10819743 | biostudies-literature | 2024 Jan-Feb

REPOSITORIES: biostudies-literature