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Ad5-nCoV Vaccination Could Induce HLA-E Restricted CD8⁺ T Cell Responses Specific for Epitopes on Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein.

ABSTRACT: We evaluated cellular immune responses induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines in an immunized population based on HLA-E-restricted CD8⁺ T cell epitope identification. HLA-E-restricted SARS-CoV-2 CD8⁺ T cell nonamer peptides were predicted with software. An HLA-E-transfected K562 cell binding assay was used to screen for high-affinity peptides. IFN-γ enzyme-linked immunospot assays were used to identify HLA-E-restricted epitopes. An HLA-E/epitope tetramer was employed to detect the frequencies of epitope-specific CD8⁺ T cells. Four CD8⁺ T cell epitopes on the spike protein of SARS-CoV-2 restricted by both HLA-E*0101 and E*0103 were identified. HLA-E-restricted epitope-specific IFN-γ-secreting CD8⁺

SUBMITTER: Wang Y

PROVIDER: S-EPMC10820189 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Ad5-nCoV Vaccination Could Induce HLA-E Restricted CD8⁺ T Cell Responses Specific for Epitopes on Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein.

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Ad5-nCoV Vaccination Could Induce HLA-E Restricted CD8+ T Cell Responses Specific for Epitopes on Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Ad5-nCoV Vaccination Could Induce HLA-E Restricted CD8⁺ T Cell Responses Specific for Epitopes on Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein.