High Genetic Diversity in Third-Generation Cephalosporin-Resistant <i>Escherichia coli</i> in Wastewater Systems of Schleswig-Holstein.

ABSTRACT: The spread of multidrug-resistant bacteria from humans or livestock is a critical issue. However, the epidemiology of resistant pathogens across wastewater pathways is poorly understood. Therefore, we performed a detailed comparison of third-generation cephalosporin-resistant Escherichia coli (3GCREC) from wastewater treatment plants (WWTPs) to analyze dissemination pathways. A total of 172 3GCREC isolated from four WWTPs were characterized via whole genome sequencing. Clonal relatedness was determined using multi-locus sequence typing (MLST) and core genome MLST. Resistance genotypes and plasmid replicons were determined. A total of 68 MLST sequence types were observed with 28 closely related clusters. Resistance genes to eight antibiotic classes were detected. In fluoroquinolone-r

SUBMITTER: Carlsen L 

PROVIDER: S-EPMC10820474 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature