Bayesian ¹³C-Metabolic Flux Analysis of Parallel Tracer Experiments in Granulocytes: A Directional Shift within the Non-Oxidative Pentose Phosphate Pathway Supports Phagocytosis.

ABSTRACT: The pentose phosphate pathway (PPP) plays a key role in the cellular regulation of immune function; however, little is known about the interplay of metabolic adjustments in granulocytes, especially regarding the non-oxidative PPP. For the determination of metabolic mechanisms within glucose metabolism, we propose a novel set of measures for ¹³C-metabolic flux analysis based on ex vivo parallel tracer experiments ([1,2-¹³C]glucose, [U-¹³C]glucose, [4,5,6-¹³C]glucose) and gas chromatography-mass spectrometry labeling measurements of intracellular metabolites, such as sugar phosphates and their fragments. A detailed constraint analysis showed that the permission range for net and irreversible fluxes was limited to a three-dimensional space. The over

SUBMITTER: Hogg M 

PROVIDER: S-EPMC10820746 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature