Microfluidic Single-Cell Study on Arabidopsis thaliana Protoplast Fusion-New Insights on Timescales and Reversibilities.

ABSTRACT: Plant cells are omnipotent and breeding of new varieties can be achieved by protoplast fusion. Such fusions can be achieved by treatment with poly(ethylene glycol) or by applying an electric field. Microfluidic devices allow for controlled conditions and targeted manipulation of small batches of cells down to single-cell analysis. To provide controlled conditions for protoplast fusions and achieve high reproducibility, we developed and characterized a microfluidic device to reliably trap some Arabidopsis thaliana protoplasts and induced cell fusion by controlled addition of poly(ethylene glycol) (PEG, with a molecular weight of 6000). Experiments were conducted to determine the survival rate of isolated protoplasts in our microfluidic system. Afterward, PEG-induced fusion was studie

SUBMITTER: Seidel T 

PROVIDER: S-EPMC10820889 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature