A Streamlined Method to Obtain Biologically Active TcdA and TcdB Toxins from Clostridioides difficile.

ABSTRACT: The major virulence factors of Clostridioides difficile (C. difficile) are enterotoxins A (TcdA) and B (TcdB). The study of toxins is a crucial step in exploring the virulence of this pathogen. Currently, the toxin purification process is either laborious and time-consuming in C. difficile or performed in heterologous hosts. Therefore, we propose a streamlined method to obtain functional toxins in C. difficile. Two C. difficile strains were generated, each harboring a sequence encoding a His-tag at the 3' end of C. difficile 630∆erm tcdA or tcdB genes. Each toxin gene is expressed using the P_tet promoter, which is inducible by anhydro-tetracycline. The obtained purification yields were 0.28 mg and 0.1 mg per liter for rTcdA

SUBMITTER: Sapa D 

PROVIDER: S-EPMC10821508 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature