Chitosan alleviates ovarian aging by enhancing macrophage phagocyte-mediated tissue homeostasis.

ABSTRACT:

Background

Age-related changes in the ovarian microenvironment are linked to impaired fertility in women. Macrophages play important roles in ovarian tissue homeostasis and immune surveillance. However, the impact of aging on ovarian macrophage function and ovarian homeostasis remains poorly understood.

Methods

Senescence-associated beta-galactosidase staining, immunohistochemistry, and TUNEL staining were used to assess senescence and apoptosis, respectively. Flow cytometry was employed to evaluate mitochondrial membrane potential (MMP) and apoptosis in granulosa cells lines (KGN), and macrophages phagocytosis. After a 2-month treatment with low molecular weight Chitosan (LMWC), ovarian tissues from mice were collected for comprehensive analysis.

Results

Compared wit

SUBMITTER: Shen HH 

PROVIDER: S-EPMC10821576 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature