Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS.

ABSTRACT: Quantitative information on blood metabolites can be used in developing advanced medical strategies such as early detection and prevention of disease. Monitoring bioactive lipids such as steroids, bile acids, and PUFA metabolites could be a valuable indicator of health status. However, a method for simultaneously measuring these bioactive lipids has not yet been developed. Here, we report a LC/MS/MS method that can simultaneously measure 144 bioactive lipids, including steroids, bile acids, and PUFA metabolites, from human plasma, and a sample preparation method for these targets. Protein removal by methanol precipitation and purification of bioactive lipids by solid-phase extraction improved the recovery of the targeted compounds in human plasma samples, demonstrating the importance of sa

SUBMITTER: Nakatani K 

PROVIDER: S-EPMC10821590 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature