M13 phage grafted with peptide motifs as a tool to detect amyloid-β oligomers in brain tissue.
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ABSTRACT:
SUBMITTER: Martins IM
PROVIDER: S-EPMC10821927 | biostudies-literature | 2024 Jan
REPOSITORIES: biostudies-literature
Publications
M13 phage grafted with peptide motifs as a tool to detect amyloid-β oligomers in brain tissue.
Martins Ivone M IM Lima Alexandre A de Graaff Wim W Cristóvão Joana S JS Brosens Niek N Aronica Eleonora E Kluskens Leon D LD Gomes Cláudio M CM Azeredo Joana J Kessels Helmut W HW
Communications biology 20240127 1
Oligomeric clusters of amyloid-β (Aβ) are one of the major biomarkers for Alzheimer's disease (AD). However, proficient methods to detect Aβ-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying Aβ-derived peptides on their surface preferentially interact with Aβ-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized Aβ-aggregates in hippocampus at an early age, prior to the occurrence of A ...[more]
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