Blinking characteristics of organic fluorophores for blink-based multiplexing.

ABSTRACT: Single-molecule fluorescence experiments have transformed our understanding of complex materials and biological systems. Whether single molecules are used to report on their nano-environment or provide for localization, understanding their blinking dynamics (i.e., stochastic fluctuations in emission intensity under continuous illumination) is paramount. We recently demonstrated another use for blinking dynamics called blink-based multiplexing (BBM), where individual emitters are classified using a single excitation laser based on blinking dynamics, rather than color. This study elucidates the structure-activity relationships governing BBM performance in a series of model rhodamine, BODIPY, and anthraquinone fluorophores that undergo different photo-physical and-chemical processes during bl

SUBMITTER: Seabury AG 

PROVIDER: S-EPMC10821931 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature