Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method.

ABSTRACT:

Purpose

A new high-resolution next-generation sequencing (NGS)-based method was established to type closely related European type II Toxoplasma gondii strains.

Methods

T. gondii field isolates were collected from different parts of Europe and assessed by whole genome sequencing (WGS). In comparison to ME49 (a type II reference strain), highly polymorphic regions (HPRs) were identified, showing a considerable number of single nucleotide polymorphisms (SNPs). After confirmation by Sanger sequencing, 18 HPRs were used to design a primer panel for multiplex PCR to establish a multilocus Ion AmpliSeq typing method. Toxoplasma gondii isolates and T. gondii present in clinical samples were typed with the new method. The sensitivity of the method was tested with serially diluted ref

SUBMITTER: Joeres M 

PROVIDER: S-EPMC10822014 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature