Capsular gene distribution and RAPD typing of Streptococcus agalactiae isolated from pregnant women.

ABSTRACT: Streptococcus agalactiae has different virulence factors, from which the capsule has the most significant role in the pathogenesis of this organism. We aimed to investigate the distribution of more prevalent capsular genes among different Random Amplified Polymorphic DNA (RAPD) types of S. agalactiae isolated from pregnant women. A total of 106 isolates were collected from 420 vaginal and rectal swabs obtained from pregnant women. The specimens were transferred using Todd Hewitt Broth and were cultured on a blood agar containing antibiotics. The S. agalactiae isolates were identified by the standard microbiological and biochemical tests. The genomic DNAs of S. agalactiae isolates were extracted using an extraction kit. Then, the PCR method was used to detection of the capsular genes. Moreo

SUBMITTER: Zakerifar M 

PROVIDER: S-EPMC10822826 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature