Engineering Bacillus subtilis J46 for efficient utilization of galactose through adaptive laboratory evolution.

ABSTRACT: Efficient utilization of galactose by microorganisms can lead to the production of valuable bio-products and improved metabolic processes. While Bacillus subtilis has inherent pathways for galactose metabolism, there is potential for enhancement via evolutionary strategies. This study aimed to boost galactose utilization in B. subtilis using adaptive laboratory evolution (ALE) and to elucidate the genetic and metabolic changes underlying the observed enhancements. The strains of B. subtilis underwent multiple rounds of adaptive laboratory evolution (approximately 5000 generations) in an environment that favored the use of galactose. This process resulted in an enhanced specific growth rate of 0.319 ± 0.005 h^-1, a significant increase from the 0.03 ± 0.008 h^-1 observed

SUBMITTER: Choi JW 

PROVIDER: S-EPMC10822834 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature