Production and characterization of single-chain variable fragment antibodies targeting the breast cancer tumor marker nectin-4.

ABSTRACT:

Background

Nectin-4 is a novel biomarker overexpressed in various types of cancer, including breast cancer, in which it has been associated with poor prognosis. Current literature suggests that nectin-4 has a role in cancer progression and may have prognostic and therapeutic implications. The present study aims to produce nectin-4-specific single-chain variable fragment (scFv) antibodies and evaluate their applications in breast cancer cell lines and clinical specimens.

Methods

We generated recombinant nectin-4 ectodomain fragments as immunogens to immunize chickens and the chickens' immunoglobulin genes were amplified for construction of anti-nectin-4 scFv libraries using phage display. The binding capacities of the selected clones were evaluated with the recombinant nectin

SUBMITTER: Liu CH 

PROVIDER: S-EPMC10822971 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature