An optimized method for extraction and purification of inorganic phosphate from plant material for oxygen isotope ratio analysis.

ABSTRACT: Compound-specific stable isotope ratio analysis of oxygen isotopes in inorganic phosphate can be used to study biological phosphorus cycling and the transformation processes controlling the fate of phosphorus. However, methods for extraction of inorganic phosphate from plant tissue for oxygen isotope ratio analysis are not consistent. Further, the purification into solid silver phosphate can be challenging and laborious. In this work, a detailed and optimized method to provide a more consistent, easily implementable and reproducible extraction using trichloroacetic acid and subsequent purification of inorganic phosphate from plant material for oxygen isotope ratio analysis is presented. Key focus points were: uniform extraction of inorganic phosphate from barley leaves, removal of dissolve

SUBMITTER: Rieckmann MM 

PROVIDER: S-EPMC10823127 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature