LincR-PPP2R5C Promotes Th2 Cell Differentiation Through PPP2R5C/PP2A by Forming an RNA-DNA Triplex in Allergic Asthma.

ABSTRACT:

Purpose

The roles and mechanisms of long noncoding RNAs (lncRNAs) in T helper 2 (Th2) differentiation from allergic asthma are poorly understood. We aimed to explore a novel lncRNA, LincR-protein phosphatase 2 regulatory subunit B' gamma (PPP2R5C), in Th2 differentiation in a mouse model of asthma.

Methods

LincR-PPP2R5C from RNA-seq data of CD4⁺ T cells of asthma-like mice were validated and confirmed by quantitative reverse transcription polymerase chain reaction, northern blotting, nuclear and cytoplasmic separation, and fluorescence in situ hybridization (FISH). Lentiviruses encoding LincR-PPP2R5C or shRNA were used to overexpress or silence LincR-PPP2R5C in CD4⁺ T cells. The interactions between LincR-PPP2R5C and PPP2R5C were explored with we

SUBMITTER: Ji N 

PROVIDER: S-EPMC10823138 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature