A TeZla micromixer for interrogating the early and broad folding landscape of G-quadruplex via multistage velocity descending.

ABSTRACT: Biomacromolecular folding kinetics involves fast folding events and broad timescales. Current techniques face limitations in either the required time resolution or the observation window. In this study, we developed the TeZla micromixer, integrating Tesla and Zigzag microstructures with a multistage velocity descending strategy. TeZla achieves a significant short mixing dead time (40 µs) and a wide time window covering four orders of magnitude (up to 300 ms). Using this unique micromixer, we explored the folding landscape of c-Myc G4 and its noncanonical-G4 derivatives with different loop lengths or G-vacancy sites. Our findings revealed that c-Myc can bypass folding intermediates and directly adopt a G4 structure in the cation-deficient buffer. Moreover, we found that the loop length and

SUBMITTER: Li Z 

PROVIDER: S-EPMC10823215 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature