Molecular features of luminal breast cancer defined through spatial and single-cell transcriptomics.

ABSTRACT:

Background

Intratumour heterogeneity is a hallmark of most solid tumours, including breast cancers. We applied spatial transcriptomics and single-cell RNA-sequencing on patient-derived xenografts (PDXs) to profile spatially resolved cell populations within oestrogen receptor-positive (ER⁺ ) breast cancer and to elucidate their importance in oestrogen-dependent tumour growth.

Methods

Two PDXs of 'ER-high' breast cancers with opposite oestrogen-mediated growth responses were investigated: oestrogen-suppressed GS3 (80-100% ER) and oestrogen-dependent SC31 (40-90% ER) models. The observation was validated via single-cell analyses on an 'ER-low' PDX, GS1 (5% ER). The results from our spatial and single-cell analyses were further supported by a public ER⁺ bre

SUBMITTER: Yoshitake R 

PROVIDER: S-EPMC10823285 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature