Full-length isoform concatenation sequencing to resolve cancer transcriptome complexity.

ABSTRACT:

Background

Cancers exhibit complex transcriptomes with aberrant splicing that induces isoform-level differential expression compared to non-diseased tissues. Transcriptomic profiling using short-read sequencing has utility in providing a cost-effective approach for evaluating isoform expression, although short-read assembly displays limitations in the accurate inference of full-length transcripts. Long-read RNA sequencing (Iso-Seq), using the Pacific Biosciences (PacBio) platform, can overcome such limitations by providing full-length isoform sequence resolution which requires no read assembly and represents native expressed transcripts. A constraint of the Iso-Seq protocol is due to fewer reads output per instrument run, which, as an example, can consequently affect the detection

SUBMITTER: Wijeratne S 

PROVIDER: S-EPMC10823626 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature