Genome-wide screen identifies new set of genes for improved heterologous laccase expression in Saccharomyces cerevisiae.

ABSTRACT: The yeast Saccharomyces cerevisiae is widely used as a host cell for recombinant protein production due to its fast growth, cost-effective culturing, and ability to secrete large and complex proteins. However, one major drawback is the relatively low yield of produced proteins compared to other host systems. To address this issue, we developed an overlay assay to screen the yeast knockout collection and identify mutants that enhance recombinant protein production, specifically focusing on the secretion of the Trametes trogii fungal laccase enzyme. Gene ontology analysis of these mutants revealed an enrichment of processes including vacuolar targeting, vesicle trafficking, proteolysis, and glycolipid metabolism. We confirmed that a significant portion of these mutants also showed increased

SUBMITTER: Strawn G 

PROVIDER: S-EPMC10823697 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature