Dopamine modification of glycolytic enzymes impairs glycolysis: possible implications for Parkinson's disease.

ABSTRACT:

Background

Parkinson's disease (PD), a chronic and severe neurodegenerative disease, is pathologically characterized by the selective loss of nigrostriatal dopaminergic neurons. Dopamine (DA), the neurotransmitter produced by dopaminergic neurons, and its metabolites can covalently modify proteins, and dysregulation of this process has been implicated in neuronal loss in PD. However, much remains unknown about the protein targets.

Methods

In the present work, we designed and synthesized a dopamine probe (DA-P) to screen and identify the potential protein targets of DA using activity-based protein profiling (ABPP) technology in combination with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In situ pull-down assays, cellular thermal shift assays (CETSAs) and immun

SUBMITTER: Chen B 

PROVIDER: S-EPMC10823740 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature