Epigenetic changes in pyloric caeca of Atlantic salmon fed diets containing increasing levels of lipids and choline.

ABSTRACT: An earlier study of ours investigating the effect of dietary lipid levels on the choline requirement of Atlantic salmon showed increasing severity of intestinal steatosis with increasing lipid levels. As choline is involved in epigenetic regulation by being the key methyl donor, pyloric caeca samples from the study were analysed for epigenetic effects of dietary lipid and choline levels. The diets varied in lipid levels between 16% and 28%, and choline levels between 1.9 and 2.3 g/kg. The diets were fed for 8 weeks to Atlantic salmon of 25 g of initial weight. Using reduced representation bisulfite sequencing (RRBS), this study revealed that increasing dietary lipid levels induced methylation differences in genes involved in membrane transport and signalling pathways, and in microRNAs impo

SUBMITTER: Dhanasiri AKS 

PROVIDER: S-EPMC10824149 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature