Exploring the expression and clinical significance of the miR-140-3p-HOXA9 axis in colorectal cancer.

ABSTRACT:

Purpose

This study aims to investigate the expression patterns and clinical significance of miR-140-3p and homeobox A9 (HOXA9) in colorectal cancer (CRC) selected by bioinformatic study, while elucidating their potential interplay.

Methods

The microRNA expression profiles of paired colorectal cancer and matched normal tissues were retrieved from the Gene Expression Omnibus Database. Differentially expressed microRNAs and microRNA candidates were filtered and subjected to further analysis. Clinicopathological data, along with paraffin-embedded samples of colorectal tumor tissues were collected to facilitate comprehensive analysis. Expression levels of miR-140-3p and HOXA9 were quantified using qRT-PCR and immunohistochemistry. Survival rates were determined using the Kaplan-M

SUBMITTER: Cui W 

PROVIDER: S-EPMC10824855 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature