Characterization of viscerofugal neurons in human colon by retrograde tracing and multi-layer immunohistochemistry.

ABSTRACT:

Background and aims

Viscerofugal neurons (VFNs) have cell bodies in the myenteric plexus and axons that project to sympathetic prevertebral ganglia. In animals they activate sympathetic motility reflexes and may modulate glucose metabolism and feeding. We used rapid retrograde tracing from colonic nerves to identify VFNs in human colon for the first time, using ex vivo preparations with multi-layer immunohistochemistry.

Methods

Colonic nerves were identified in isolated preparations of human colon and set up for axonal tracing with biotinamide. After fixation, labeled VFN cell bodies were subjected to multiplexed immunohistochemistry for 12 established nerve cell body markers.

Results

Biotinamide tracing filled 903 viscerofugal nerve cell bodies (n = 23)

SUBMITTER: Chen BN 

PROVIDER: S-EPMC10825022 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature