AMPK activation enhances osteoblast differentiation on a titanium disc via autophagy.

ABSTRACT:

Purpose

The acquisition of osseointegration during implant therapy is slower and poorer in patients with diabetes compared with healthy persons. The serum concentration of adiponectin in patients with type II diabetes is lower than that of healthy persons via the suppression of AMP-activated protein kinase (AMPK). Therefore, we hypothesized that the AMPK activation enhances bone formation around implants, resulting in the improved acquisition of osseointegration. The purpose of this study was to evaluate the impact of AMPK activation on osteoblast differentiation and its mechanism of downstream signaling on titanium disc (Ti).

Methods

Confluent mouse pre-osteoblasts (MC3T3-E1) cells (1 × 10⁵ cells/well) were cultured with BMP-2 for osteoblast differentiation, in t

SUBMITTER: Egashira K 

PROVIDER: S-EPMC10825085 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature