Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR.

ABSTRACT: Microglia represent the primary immune defense system within the central nervous system and play a role in the inflammatory processes occurring in numerous disorders, such as Parkinson's disease (PD). PD onset and progression are associated with factors considered possible causes of neuroinflammation, i.e. genetic mutations. In vitro models of microglial cells were established to identify specific molecular targets in PD through the analysis of gene expression data. Recently, the Human Microglial Clone 3 cell line (HMC3) has been characterized and a new human microglia model has emerged. Here we perform RT-qPCR analyses to evaluate the expression of ten reference genes in HMC3, untreated or stimulated to a pro-inflammatory status. The comparative ∆C_T method, BestKeeper, Normfind

SUBMITTER: Fazzina M 

PROVIDER: S-EPMC10825209 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature