Proteomic analysis of the extracellular matrix of human atherosclerotic plaques shows marked changes between plaque types.

ABSTRACT: Cardiovascular disease is the leading cause of death, with atherosclerosis the major underlying cause. While often asymptomatic for decades, atherosclerotic plaque destabilization and rupture can arise suddenly and cause acute arterial occlusion or peripheral embolization resulting in myocardial infarction, stroke and lower limb ischaemia. As extracellular matrix (ECM) remodelling is associated with plaque instability, we hypothesized that the ECM composition would differ between plaques. We analyzed atherosclerotic plaques obtained from 21 patients who underwent carotid surgery following recent symptomatic carotid artery stenosis. Plaques were solubilized using a new efficient, single-step approach. Solubilized proteins were digested to peptides, and analyzed by liquid chromatography-mass

SUBMITTER: Lorentzen LG 

PROVIDER: S-EPMC10825564 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature