Diversity of transcripts of nitrite reductase genes (nirK and nirS) in rhizospheres of grain legumes.

ABSTRACT: Transcription of the nirK and nirS genes coding for dissimilatory bacterial nitrite reductases was analyzed by reverse transcription PCR (RT-PCR) of mRNA isolated from rhizosphere samples of three economically important grain legumes at maturity: Vicia faba, Lupinus albus, and Pisum sativum. The nirK gene and transcripts could be detected in all the rhizosphere samples. In contrast, nirS could not be detected. Sampling variations were analyzed by comparing denaturing gradient gel electrophoresis profiles derived from nirK RT-PCR products. High similarity was observed between the replicates, and so one representative product per legume was cloned. Clones with the correct insert size were screened by restriction fragment length polymorphism by using the restriction enzyme MspI. The clones co

SUBMITTER: Sharma S 

PROVIDER: S-EPMC1082563 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature