Distinct spatial landscapes in clear-cell renal cell carcinoma as revealed by whole transcriptome analysis.

ABSTRACT:

Background

Clear-cell renal cell carcinoma (ccRCC) is the most common and aggressive form of renal cancer and a paradigm of inter- and intratumor heterogeneity. We carried out an exploratory digital spatial profiling of the tumor interior and periphery of two ccRCC tumor specimens and mapped spatially the molecular and cellular composition of their tumor microenvironment and ecosystem.

Materials and methods

Digital spatial profiling of the whole transcriptome of 19 regions of interest (ROIs) was carried out from two selected highly immunogenic stage pT3a/grade 3 (G3) and stage pT3a/grade 4 (G4) ccRCC. A total of 9-10 ROIs were selected from distinct areas from each tumor, including tumor interior and tumor periphery, and differences in gene expression were analyzed by RNA se

SUBMITTER: Lopez JI 

PROVIDER: S-EPMC10825646 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature