Single-cell RNA-seq mapping of chicken peripheral blood leukocytes.

ABSTRACT:

Background

Single-cell transcriptomics provides means to study cell populations at the level of individual cells. In leukocyte biology this approach could potentially aid the identification of subpopulations and functions without the need to develop species-specific reagents. The present study aimed to evaluate single-cell RNA-seq as a tool for identification of chicken peripheral blood leukocytes. For this purpose, purified and thrombocyte depleted leukocytes from 4 clinically healthy hens were subjected to single-cell 3' RNA-seq. Bioinformatic analysis of data comprised unsupervised clustering of the cells, and annotation of clusters based on expression profiles. Immunofluorescence phenotyping of the cell preparations used was also performed.

Results

Computational analysis

SUBMITTER: Maxwell M 

PROVIDER: S-EPMC10826067 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature